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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Cancer Res. 2009 Feb 10;69(4):1536–1544. doi: 10.1158/0008-5472.CAN-08-3718

Figure 4. RhoB upregulation by RS5444 occurs prior to p21 upregulation.

Figure 4

A. Real time PCR and westerns for p21 expression in all four cell lines showed p21 induction after 10 nM RS5444 treatment for 24 hrs. Fold change values were calculated between treated and control samples and plotted as mean ± S.D; n=3. * indicates p<0.01 when compared to control. B. Real time PCR of RhoB mRNA in DRO cells upon exposure to RS5444 showed induction as early as 2 hrs. C. Real time PCR of p21 mRNA in DRO cells demonstrated induction by 4 hrs. Data was normalized to 18S and plotted as mean ± SD with n = 4. * indicates p<0.05 when compared to control. D. Immunoblotting of DRO cells treated with RS5444 demonstrated upregulation of RhoB protein by 4 hours and p21 protein by 6 hrs. No change in RhoA protein occurred between control and RS5444 treated cells.