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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Cancer Res. 2009 Feb 10;69(4):1536–1544. doi: 10.1158/0008-5472.CAN-08-3718

Figure 5. RhoB upregulation by RS5444 is required to induce p21.

Figure 5

A. Real time PCR of p21 mRNA in DRO and KTC2 illustrates p21 mRNA's dependence upon RhoB induction when stimulated by RS5444. Cells were transiently transfected and treated as previously described. Data was plotted as fold change of p21 mRNA expression as compared to scrambled control ± S.D with n = 3. * indicates p<0.05 when compared to untreated control. + indicates p<0.05 when compared to RhoB siRNA control or to scrambled siRNA with RS5444. B. Real time PCR of p21 mRNA using dominant negative (DN) RhoB also demonstrated p21 mRNA's dependence upon RhoB induction through PPARγ. Cells were transiently transfected with either pcDNA3.1 or DN RhoB and then treated with RS5444 for 24 hrs. C. Silencing RhoB blocked RS5444 stimulated p21 protein expression in transiently transfected DRO and KTC2 cells. Protein lysates were examined for RhoB and p21 expression. RhoA expression was examined to demonstrate specificity of the RhoB siRNA.