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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2008 Nov 26;4(1):116–128. doi: 10.1007/s11481-008-9140-4

Figure 6. Expression and FRET analysis of ECFP-OTK18-EYFP.

Figure 6

A, The cells were transfected with ECFP-OTK18-EYFP and treated with 10 mM MG132 for the times indicated. Total cell extract was subjected to immunoblotting using anti-OTK18 mAb. B, The cells were transfected with ECFP, EYFP, or ECFP-OTK18-EYFP 24 hours prior to imaging, and excited with CFP filter (CFPex for ECFP, EYFP, or ECFP-OTK18-EYFP) or YFP filter (EYFP only), and images were taken by CFP filter (CFPem, left panel) and YFP filter (YFPem, right panel) using Dual-View imaging system. Original magnification: 400X.