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(A) SDS–PAGE analysis. Lane 1, p2B3 scFv; lane 2, bm2B3 scFv; lane 3, p2B3-Db8; lane 4, bm2B3-Db8; lane 5, p2B3-Db5 and lane 6, bm2B3-Db5. (B) Indirect flow cytometry to study the effect of back-mutations on binding to PSCA-SKW 6.4 cells. For the negative control, only mouse anti-His6-tag antibody and PE-conjugated goat anti-mouse (Fc-specific) antibodies were used.
