Fig. 2.

Mean pericardium size, based on 2D pixel area, was used to investigate pericardial effusion in 96 hpf zebrafish embryos. Embryos were dosed at 24 hpf with either (A) 10 µg L⁻¹ BaP, and/or 500 µg L⁻¹ FL; or (B) 1 µg L⁻¹ BNF and/or 100 µg L⁻¹ ANF. Embryos were then exposed to either normoxia (20.8% DO) or hypoxia (7.3% DO). DMSO was used as the carrier control in all experiments. These data show that while hypoxia is capable of significantly reducing BaP-induced CYP1A induction (Fig. 1), this reduction does not lead to an increase in pericardial effusion as seen with FL or ANF. Groups not sharing a common letter are significantly different (p ≤ 0.05), based on Bonferroni-corrected post hoc multiple comparisons following ANOVA.