Figure 4.
Skeletal muscle specific deletion of CnB1 increases average fiber area, decreases centrally-nucleated fibers and ameliorates fibrosis in scgd−/− mice. (A) Representative Masson's trichrome staining of paraffin sections from diaphragm of age-matched scgd−/− and scgd−/− CnB1fl/flMLC-cre mice. (B) Analysis of average muscle fiber area in the diaphragm of Wt, scgd−/− and scgd−/− CnB1fl/flMLC-cre mice. The number of muscles assayed is shown for each group in the panel. *P<0.05 versus Wt; †P<0.05 versus scgd−/−. (C) Quantitation of fibers containing central nuclei in the diaphragm of Wt, scgd−/− and scgd−/− CnB1fl/flMLC-cre mice. The number of muscles assayed is shown for each group in the panel. *P<0.05 versus Wt; †P<0.05 versus scgd−/−. (D) Analysis of μg hydroxyproline / mg of tissue for the indicated muscle groups from Wt, CnB1fl/flMLC-cre, scgd−/−, and scgd−/− CnB1fl/flMLC-cre mice. The number of muscles assayed is shown for each group in the panel. *P<0.05 versus Wt; †P<0.05 versus scgd−/−.