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. Author manuscript; available in PMC: 2009 Feb 18.
Published in final edited form as: Biol Blood Marrow Transplant. 2008 Jan;15(1 Suppl):33–38. doi: 10.1016/j.bbmt.2008.10.027

Fig 5. Intact Protein Analysis System workflow and in-depth analysis of plasma proteins.

Fig 5

Plasma pooled from ten patients with GVHD are labeled with the heavy isotope and compared to plasma pooled from ten patients with no GVHD labeled with the light isotope. The specimens are then subjected to extensive fractionation (by ion-exchange chromatography and reversed-phase chromatography) before analysis of individual fractions. The result is a reduced complexity of individual fractions subjected to analysis by liquid chromatography – tandem mass spectrometry (LC-MS/MS). Adapted from reference 32.