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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Dev Cell. 2008 Dec;15(6):890–900. doi: 10.1016/j.devcel.2008.10.003

Figure 1. An NH2-terminal motif in E2f1 mediates S phase-specific destruction.

Figure 1

(A) Cell cycle profiles of S2 cells stably transfected with Actin5C-GFP or Actin5C-GFP-E2f1. DNA content profile of GFP positive cells (gray area: defined where 99.9% of non-transfected cells are excluded) is overlaid on the profile of all cells in the population (unfilled solid line). The Y axis is shown as a relative scale between “all cells” and “GFP-positive cells”. Insets show percentages of S phase cells for the entire population and for GFP-positive cells. (B) Schematic of E2f1 mutants analyzed by FACS. + and − indicates S phase-specific destruction of GFP-E2f1 (WT) and mutant variants. Destruction motif within residues 139–184 is shadowed. PIP=PCNA-interaction protein box. DNA=DNA-binding domain. Dp=Dp-dimerization domain. MB=marked box. TA&Rbf=transactivation and Rbf1 binding domain. (C) Cell cycle profiles of S2 cells transiently transfected with plasmids expressing Actin5C-GFP-E2f1 variants.