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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Dev Cell. 2008 Dec;15(6):890–900. doi: 10.1016/j.devcel.2008.10.003

Figure 3. RNAi analysis of E2f1 destruction.

Figure 3

(A) Hsp70-GFP-E2f1 cells were treated with the indicated dsRNAs for 2 days and analyzed by FACS 225 min after a 30 min at 37° C heat shock. (B) mRNA levels of RNAi-targeted genes was analyzed by RT-PCR. RT indicates the presence (+) or absence (−) of reverse transcriptase. rp49 is a ubiquitously expressed control. RNAi for Cul4, Ddb1, Cdt2, or Pcna does not affect the other genes of the Cul4 complex. (C) Western blot of the RNAi-treated cells. α-Tubulin is a loading control. (D) Diagram of the Cul4Cdt2 E3 ligase predicted to target E2f1.