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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Dev Cell. 2008 Dec;15(6):890–900. doi: 10.1016/j.devcel.2008.10.003

Figure 4. Mutations in the PIP box stabilize E2f1 during S phase in vivo.

Figure 4

(A) Stage 11 en-Gal4>UAS-GFP-E2f1 or en-Gal4>UAS-GFP-E2f1PIP-3A embryos were stained for GFP (green), BrdU incorporation (red), and phosphotyrosine (P-Tyr, blue) to visualize cell boundaries. Brackets indicate cells undergoing S phase that overlap with the dorsal portion of the en-Gal4 expressing cells. (B) 3rd instar en-Gal4>UAS-GFP-E2f1 or en-Gal4>UAS-GFP-E2f1PIP-3A wing discs were stained for GFP (green) and BrdU incorporation (red). Green and red arrows indicate cells that are positive only for GFP-E2f1 or BrdU, respectively. Yellow arrows indicate GFP-E2f1PIP-3A and BrdU double positive cells. Scale bars are 20 μm.