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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Dev Cell. 2008 Dec;15(6):890–900. doi: 10.1016/j.devcel.2008.10.003

Figure 7. Stabilization of E2f1 during S phase induces apoptosis.

Figure 7

(A) 3rd instar en-Gal4>UAS-GFP-E2f1 or en-Gal4>UAS-GFP-E2f1PIP-3A larvae wing discs were stained for cleaved Caspase-3 (red) to detect apoptosis and DNA (DAPI). Anterior: top left. Posterior: bottom right. Caspase-3-positive cells are extruded from the surface of the disc, as evident by the DAPI staining image of apical confocal sections. Scale bar is 50 μm. (B) Wings of adult flies eclosed from en-Gal4>UAS-GFP-E2f1 or en-Gal4>UAS-GFP-E2f1PIP-3A cultures. The percentages indicate the fraction of total wings scored. Note that 5% of the en-Gal4>GFP-E2f1PIP-3A wings appeared normal.