Table 4.
mRNA Expression of Enzymes in Liver and Adipose Tissue Following Dietary Interventions (Means±SE)
| High Fat | High Sucrose | |||
|---|---|---|---|---|
| LD | HD | LD | HD | |
| Liver CPT-1ab | 1.00±0.24 | 1.51±0.24 | 1.00±0.21 | 1.19±0.35 |
| Liver FASc | 1.00±0.32 | 0.82±0.09 | 1.00±0.10 | 1.46±0.21 |
| Liver PEPCKd | 1.00±0.36 | 0.53±0.11 | 1.00±0.35 | 1.44±0.38 |
| Liver PGC-1αe | 1.00±0.23 | 1.44±0.40 | 1.00±0.10 | 1.08±0.09 |
| Liver UCP2f | 1.00±0.12 | 0.79±0.12 | 1.00±0.09 | 0.89±0.13 |
| Adipose UCP2 | 1.00±0.19 | 1.00±0.14 | 1.00±0.20 | 0.91±0.16 |
| Adipose UCP3 | 1.00±0.15 | 0.82±0.15 | 1.00±0.15 | 1.20±0.19 |
The diets were based on the AIN 93G diet with either high fat with 40% of energy primarily from soybean oil or high sucrose with 66% of energy primarily from sucrose. Low fat and high fat diets contained low whey, low calcium and low vitamin D (LD) or high whey, high calcium and high vitamin D (HD).
Abbreviations are carnitine palmitoyltransferase-1 (CPT-1), fatty acid synthase (FAS), phosphoenolpyruvate carboxykinase (PEPCK), peroxisome proliferative activated receptor γ coactivator-1α (PGC-1α), uncoupling protein (UCP). Data is expressed over LD average for background diet. Statistical analyses compared LD and HD within dietary background (high fat or high sucrose) by two tailed T test.