Fig. 7.

The effect of pretreatment with NAC on apoptosis in CdCl₂ treated cells. Cells were treated with 10 μM CdCl₂ only or pretreated with 1 mM NAC followed by 48 h exposure to 10 μM CdCl₂. Controls received culture medium only. Apoptosis was determined using APOPercentage dye which is transported into an apoptotic cell during the translocation of phosphatidylserine from the inner leaflet to the outer leaflet of the cell membrane. (A) Images of pink stained (apoptotic) cells and (B) quantification of APOPercentage dye uptake. Each bar represents the mean ± SEM of at least three independent experiments. ^*Denotes significant difference from control p < 0.05.