Table 2.

Peptide Sequences of Ku70

Sequence	Location in Ku70
DSLIFLVDASK	36–46
ILELDQFK	116–123
DIISIAEDEDLR	219–230

The nuclear extracts of Huh-7 cells were preincubated with avidin-agarose beads (Pierce, Rockford, IL), followed by incubation with the biotinylated163-bp ApoC-IV promoter immobilized on avidin-agarose beads. Bound proteins were separated in 0.1% SDS-8% PAGE and revealed by silver staining (Invitrogen, Carlsbad, CA). The protein bands were excised and sequenced at the Proteomic Core Facility of the Rhode Island Hospital.