Figure 6.

Loss of Pkd1 does not constitutively activate the mTOR pathway. MEFs were serum deprived overnight, then stimulated for 15 min in the presence of 20% FBS. Immunoblots were probed with phospho-S6 to determine the level of mTOR activation. β-actin was used as a loading control. (A) Western immunoblot of Tsc2^−/−p53^−/−, Pkd1^+/+p53kd MEFs and Pkd1^−/−p53kd MEFs. (B) Western immunoblot of Tsc2^−/−p53^−/−, Pkd1^−/− primary MEFs and Pkd1^−/−p53kd MEFs. Similar results were seen in four independent experiments.