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MAPK activation in ubiquitous LEOPARD and Noonan syndrome-transgene expressing larvae. Immunoblots of protein lysates from second instar larvae using antibodies against dpERK (activated MAPK) and ERK (all forms of MAPK). ERK is expressed at approximately equal levels in all fly lines. The expression of dpERK is increased in the wild-type and mutant transgenic larvae, as compared to the wild-type larvae showing a minimal level of dpERK.
