Figure 2.

Immunohistochemical analysis of sapje-like horizontal sections at five days post-fertilization (dpf). Dystrophin protein localizes in unaffected siblings to the muscle myosepta (top left), but is absent in the mutant (top right). γ-Sarcoglycan is also strongly expressed in unaffected siblings (bottom left) and also in the sapje-like mutant (bottom right). From this analysis, it is difficult to determine if γ-sarcoglycan is down-regulated in the mutant. The myoseptas are designated with white arrows. For each antibody, pictures were taken at the same exposure level for both the wild-type and sapje-like mutant. All panels are shown at approximately 80-fold magnification.