Figure 3.

Gel band protein identifications and their relationship with theoretical molecular weight. The right hand bar chart shows of the number of protein identifications made from each gel slice using the average peptide scoring method when searching the DSM A. niger database. Dark green sections represent protein identifications unique to that gel band. Subsequent colours, shown in the key at the top of the plot, indicate the distance in consecutive gel bands that a particular set of protein identifications are shared i.e. red shows protein identifications which are found more than 10 bands from the current positions. The left hand bar chart shows the mean protein mass in KDa for proteins identified in each gel band. As should be expected the average protein mass decreases from the top to the bottom of the gel.