Table 2.
The fifty most abundant transcripts found in the combined unfed/2-d fed and 6-d fed D. variabilis midgut libraries.
| Transcript No. | No. of EST | Representative EST† | Best match to NR protein database | E-value | Genbank | Putative Function |
| 2 | 480 | B04_DVMGL_P10 | similar to Plasmodium falciparum | 1.3 | AAO51426 | mitochondrial DNA* |
| 4 | 36 | A09_DVMGS_P8 | hypothetical protein [A. thaliana] | 0.035 | AAG50693 | Unknown |
| 5 | 28 | B11_DVMGM_P11 | ML domain-containing protein [I. ricinus] | 1E-028 | AAP84098 | ML domain-containing |
| 6 | 22 | DVMG2M-P6_C01 | cytochrome c oxidase I [R. sanguineus] | 1E-149 | NP_008511 | cytochrome oxidase 1 |
| 7 | 19 | DVMG2L-P12_F09 | cytochrome c oxidase III [R. sanguineus] | 1E-106 | NP_008515 | cytochrome oxidase 3 |
| 8 | 16 | F04_DVMGL_P10 | salivary secreted protein [I. scapularis] | 5E-019 | AAY66581 | salivary secreted protein |
| 1 | 14 | D04_DVMGM_P3 | mitochondrial* | |||
| 9 | 13 | DVMG2S-P4_E04 | mitochondrial* | |||
| 11 | 9 | G06_DVMGM_P11 | hypothetical protein [S. dysenteriae] | 0.77 | YP_405617 | mitochondrial* |
| 10 | 9 | C06_DVMGM_P12 | glutathione S-transferase [D. variabilis] | 2E-082 | AAO92279 | Glutathione S-transferase |
| 16 | 8 | DVMG2S-P3_B05 | ATP synthase F0 subunit 6 [R. sanguineus] | 8E-051 | NP_008514 | ATPase 6 |
| 15 | 8 | DVMG2L-P9_H04 | NADH dehydrogenase subunit 1 [R. sanguineus] | 1E-061 | NP_008517 | NADH dehydrogenase 1 |
| 19 | 7 | DVMG2L-P12_F02 | cytochrome b [R. sanguineus] | 1E-144 | NP_008522 | cytochrome b |
| 18 | 7 | DVMG2M-P7_E08 | NADH dehydrogenase subunit 3 [R. sang | 2E-032 | NP_008516 | oxidoreductase |
| 17 | 7 | DVMG2S-P3_E03 | cytochrome c oxidase subunit II [R. sanguineus] | 1E-066 | NP_008512 | cytochrome oxidase 2 |
| 23 | 6 | DVMG2M-P8_D03 | hypothetical protein [P. falciparum 3D7] | 0.49 | CAB39005 | Unknown |
| 22 | 6 | DVMG2L-P11_D11 | transcription factor [C. neoformans] | 0.35 | AAW44646 | Metallothionein, predicted* |
| 21 | 6 | DVMG2S-P2_B10 | Unknown [D. rerio] | 0.057 | AAI16539 | Unknown |
| 20 | 6 | DVMG2L-P11_B01 | proline threonine rich protein [Mycobacterium] | 2E-009 | YP_637621 | Mucin characteristics |
| 27 | 5 | DVMG2S-P1_D02 | putative salivary secreted peptide [I. pacificus] | 4E-008 | AAT92122 | salivary secreted peptide |
| 26 | 5 | DVMG2M-P8_H10 | 60S ribosomal protein L13A [M. edulis] | 4E-075 | ABA46793 | 60S ribosomal protein L13 |
| 25 | 5 | DVMG2L-P9_E07 | 40S ribosomal protein S12 [D. variabilis] | 8E-071 | AAP04352 | 40S ribosomal protein S12 |
| 24 | 5 | A02_DVMGM_P11 | Phosphatidylinositol 3-4-kinase [T. thermo] | 0.60 | EAR84499 | Unknown |
| 37 | 4 | DVMG2M-P8_A01 | similar to Collagen [Mu. musculus] | 0.35 | XP_992431 | Unknown |
| 36 | 4 | DVMG2M-P7_G12 | NADH dehydrogenast 4 [R. sanguineus] | 8E-062 | NP_008519 | NADH dehydrogenase 4 |
| 33 | 4 | DVMG2L-P10_D10 | NADH dehydrogenase 2 [R. sanguineus] | 1E-019 | NP_008510 | NADH dehydrogenase 2 |
| 32 | 4 | DVMG2L-P9_G11 | Forkhead-associated [Psychrobacter sp.] | 0.016 | ZP_01272146 | Unknown |
| 29 | 4 | E08_DVMGL_P2 | guanine nucleotide-binding [I. scapularis] | 0.0 | AAY66933 | Nucleotide binding protein |
| 28 | 4 | B07_DVMGM_P11 | Salivary gland secretion [D. melanogaster] | 9E-007 | NP_523475 | Unknown |
| 12 | 4 | B01_DVMGL_P1 | cathepsin L-like [H. longicornis] | 2E-050 | BAA34704 | cathepsin L proteinase B |
| 57 | 3 | DVMG2S-P2_H02 | predicted protein [M. grisea] | 5.1 | XP_367470 | Unknown |
| 56 | 3 | DVMG2M-P8_B09 | ferritin heavy chain-1a [C. rotundicauda] | 2E-043 | AAW22506 | Ferritin |
| 55 | 3 | DVMG2S-P1_G12 | similar to 60S ribosomal protein L29 [P. troglod | 4E-021 | XP_517026 | 60S ribosomal protein L29 |
| 54 | 3 | DVMG2S-P1_A07 | ribosomal protein L44 [. pacificus] | 3E-057 | AAT92163 | ribosomal protein L44 |
| 53 | 3 | DVMG2S-P4_G09 | 39S ribosomal protein L12 [S. purpuratus] | 1E-037 | XP_790377 | mitochondrial ribosomal |
| 49 | 3 | DVMG2S-P2_B01 | 60S ribosomal protein L35 protein [I. pacificus] | 9E-057 | AAT92193 | 60S ribosomal protein L35 |
| 48 | 3 | DVMG2L-P10_G1 | 40S ribosomal protein S3a [I. scapularis] | 1E-109 | AAY66934 | 40S ribosomal protein S3a |
| 47 | 3 | DVMG2L-P9_F10 | ribosomal protein L44 [I. pacificus] | 1E-104 | AAT01919 | ribosomal protein S3 |
| 46 | 3 | H12_DVMGL_P2 | hypothetical protein [P. troglodytes] | 2.1 | XP_525349 | Unknown |
| 45 | 3 | DVMG2S-P1_A03 | 40S ribosomal protein S10 [I. scapularis] | 6E-032 | AAY66831 | 40S ribosomal protein S10 |
| 44 | 3 | C10_DVMGM_P3 | ribosomal protein L18a [I. scapularis] | 1E-055 | AAY66898 | ribosomal protein L18a |
| 43 | 3 | DVMG2L-P9_G05 | ATP synthase c-subunit [D. variabilis] | 5E-067 | AAO92282 | ATP synthase c-subunit |
| 42 | 3 | C01_DVMGL_P2 | midgut cysteine proteinase 1 [R. append] | 1E-056 | AAO60044 | midgut cys proteinase 1 |
| 41 | 3 | F05_DVMGL_P1 | hypothetical protein [O. sativa] | 0.35 | NP_914254 | Unknown |
| 40 | 3 | DVMG2M-P7_C02 | ribosomal protein L12 [I. scapularis] | 6E-085 | AAY66840 | ribosomal protein L12 |
| 39 | 3 | A12_DVMGM_P4 | unknown | |||
| 38 | 3 | C09_DVMGL_P1 | ribosomal protein S19 [I. scapularis] | 2E-040 | AAY66936 | ribosomal protein S19 |
| 34 | 3 | DVMG2M-P6_G07 | cytochrome c oxidase I [D. variabilis] | 1E-101 | AAF61361 | cytochrome c oxidase I |
| 30 | 3 | G03_DVMGL_P10 | SAICAR synthase [S. purpuratus] | 8E-048 | XP_801851 | SAICAR synthase |
| 13 | 3 | DVMG2L-P11_E05 | cathepsin L-like [R. haemaphysaloides] | 1E-116 | AAQ16117 | cathepsin L proteinase A |
† Represents clone corresponding to the largest EST. Asterisks (*) indicate assignment of putative function based on a database other than ACARI or NR