Table 1.
Methanol(25%) | Ethanol (25%) | Glycerol (25%) | DMSO (25%) | |
---|---|---|---|---|
Vcosol./Vo | 0.83 ± 0.1 | 0.41 ± 0.06 | 1.6 ± 0.6 | 1.2 ± 0.2 |
Assays were conducted at 22.5 °C, pH 7.4 in 40 mM HEPES 0.01% (W/V) Tween® 20, 5 μM SNAPtide substrate and 200 nM enzyme. Values are the mean ± SEM of triplicate measurements.