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. 2009 Mar 3;3(3):e387. doi: 10.1371/journal.pntd.0000387

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Santos et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Ecto-ATPDase (solid bars) and Ecto-ADPDase (open bars) activities from live trypomastigotes from different cellular passages (P1, P3 and P4). P1-1 and P1-2 are the first and second massive exits of parasites from the 1^st passage; in the same way P3-1 and P3-2 are the first and second massive exits of parasites for the 3^rd passage. Data are mean±SE of triplicate assays from one experiment. The inset shows the ATPase/ADPase hydrolytic activities ratio. B) Microphotograph of infected VERO cells culture after 24 h of parasite-cell interaction at the 3^rd to 4^th passage. Spherical bodies are non-internalized amastigote-like parasites. C) Zoom from box section shown in B. Black arrow exemplifies a non-internalized amastigote-like and white arrow a non-internalized trypomastigote parasite.