Table 2. Effect of antiserum anti-NTPDase-1-HexaHIS or pre-immune serum on in vitro infectivity of trypomastigotes.
| Antiserum anti-NTPDase-1-HexaHIS | Pre-immune serum | |||||||
| 1∶50 | 1∶100 | 1∶50 | 1∶100 | |||||
| − | + | − | + | − | + | − | + | |
| # Parasites/infected cell | 2.11±0.31 | 2.04±0.50 | 1.47±0.30 | 2.20±0.13 | 5.88±0.82 | 6.40±1.30 | 5.88±0.82 | 5.34±0.76 |
| # Infected cells/300 cells | 18.9±3.22 | 8.83±1.75 | 17.4±3.53 | 8.96±1.16 | 109.0±6.62 | 135.0±17.1 | 109.0±6.62 | 122.1±11.5 |
| Infected cells inhibition (%) | 53±0.58 * | 49±0.38 * | 0 | 0 | ||||
*: Percent inhibition of infection was calculated for each inhibition assay relative to control data. Data reflect the mean±SE from three analyzed slides. Asterisks indicate significant differences (p<0.05) between the control without antiserum (−) and the tests with antiserum (+).