Fig. 2.

Cholinergic and nitrergic neurons in the rumen. Immunohistochemical detection of ChAT (A) revealed a subpopulation of all myenteric neurons in goat rumen stained against HuC/D. Neurons that were ChAT negative and HuC/D positive were labelled by NADPH-diaphorase reaction [dark stained neurons in (C), indicated by arrows]. NADPH/D-positive neurons were clustered at the margins of ganglia in goat (C) and cattle (D) or were equally distributed in the ganglia of fallow deer (E) and sheep (F).