Sex- and tissue-specific expression of alternative transcripts from six D. melanogaster genes that showed significant probe-by-sex interaction in the microarray data (solid bars, males; open bars, females). The RT–PCR results are analyzed as the average ratio of CT values for the two exon junctions amplified for each gene (y-axis), and error bars show the standard deviation. (A) Ratio of transcripts hts-RA and hts-RB, -RE, and -RF. (B) Ratio of transcripts CG6016-RA and CG6016-RB. (C) Ratio of transcripts CG4662-RA and CG-4662-RB. (D) Ratio of transcripts ltd-RB and ltd-RC,-RE. (E) Ratio of transcripts garz-RA and garz-RB. (F) Ratio of transcripts jdp-RA and jdp-RC.