Figure 4.—

IME2-ΔC241 exacerbates the smk1-2 sporulation phenotype. The indicated strains were pregrown in YEPD, sporulated, stained with DAPI, and photographed using phase-contrast microscopy. All strains are homozygous except for the IME2-ΔC241/IME2 strain in F as indicated. Note the predominance of asci containing four refractile spores formed in the smk1-2 strain at the semipermissive temperature (arrows in C), the asci containing two refractile spores and two nuclei that are not contained in spore walls in the IME2-ΔC241/IME2-ΔC241 strain (arrows in D), and asci containing four meiotic products and the absence of refractile spores in the homozygous IME2-ΔC241/IME2-ΔC241 smk1-2/smk1-2 strain (arrows in E).