Figure 3.—

Reduced dopamine and serotonin content. Monoamine levels were measured using HPLC. (A) Dopamine (pg/head): reduced dopamine content compared to the control lines CS (+/+) and w¹¹¹⁸CS₁₀ (w⁻; +/+) was seen in flies heterozygous for dVMAT^P1 (P/+), dVMAT^Δ14 (D14/+), and two genetic deletions CX1/+ and MK2/+. More severe reductions were seen in the homozygous mutants P/P and D14/D14, as well as in P/CX1 and P/MK2. The reduction in dopamine levels in P/P can be partially rescued by daughterless driving the expression of UAS-DVMAT-A cDNA (P/P; rescue/+). Significance values for the rescue line “P/P, rescue/+” compared to P/P or controls are indicated (one-way ANOVA P < 0.0001. Dunnett's post-test, **P < 0.01). (B) Serotonin (pg/head): serotonin levels are reduced in homozygous mutants and are not rescued by the expression of DVMAT-A cDNA using the da-GAL4 driver. (one-way ANOVA P < 0.0001, Bonferroni multiple comparison post-test separated two groups as indicated, ***P ≤ 0.001).