Figure 1.—

Salivary gland development visualized using fkhGal4-driven GFP-marker expression. Salivary gland morphogenesis from embryonic stages 10–15. (A) Schematic of salivary gland invagination, ventral view. (B–F) Low-magnification confocal sections of embryos stained with phalloidin to reveal actin (red) and expressing GFP-EFGas2 under the control of fkhGal4 in the salivary glands (green). (C–E) Lateral views. (B) A ventral view. (F) A dorsal view. (G–K′) Close-up confocal sections of salivary glands labeled with phalloidin to reveal actin (red) and expressing SrcGFP under the control of fkhGal4 (green, and as a single channel in G′–K′). G–K show lateral views. Note that GFP-EFGas2 labels microtubules, whereas SrcGFP is targeted to the membrane and thus reveals cell shape.