Figure 9.—

The bmr6-ref, but not the bmr6-3 mutation, causes downregulation of SbCAD2 expression in 2-week-old plants. (Top lane) Products from RT–PCR with primers specific for a fragment of the cDNA encoding SbCAD2. (A) 1–4, N3 cDNA; 5–8, bmr6-3 cDNA. (B) 1–6, BTx623 cDNA; 7–12, BTx623 bmr6-ref cDNA. Odd numbers correspond to cDNA obtained from shoots; even numbers cDNA obtained from roots. (Bottom lane) Products from RT–PCR with primers for Ubiquitin gene as a cDNA loading control. The size marker is Bioline Hyperladder II (cat. no. 33054).