Figure 2.

SfIAP and CpIAP protect cells against Bax- but not Fas-induced apoptosis. Expression plasmids encoding Bax (A) or Fas (B) were cotransfected into 293 cells with the indicated myc-tagged IAP expression plasmids. Percentage apoptosis was measured 24–36 hr later by 4′-6-diamidino-2-phenylindole staining (mean ± SD; n = 3). (C) Lysates were prepared from 293 cells 1 day posttransfection, normalized for total protein content (50 μg), and analyzed by SDS/PAGE–immunoblotting by using anti-myc antibody with enhanced chemiluminescence-based detection to demonstrate that the desired protein was expressed in each case.