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. Author manuscript; available in PMC: 2009 Dec 20.
Published in final edited form as: Virology. 2008 Oct 21;382(2):226–238. doi: 10.1016/j.virol.2008.09.019

FIGURE 3. Pathogenicity and immunogenicity of recombinant RV in the primary response to immunization.

FIGURE 3

Mice were immunized intranasally with 1.5×105 ffu of sucrose purified RV. (A) Survivorship of the mice was monitored by a Kaplan-Meier test for significance. We saw a significant increase in the survival of mice immunized intranasally with IFN(+) as compared to those immunized with IFN(−). (B) Viral messenger RNA recovered from the brains of infected mice was quantified using real time PCR at 8, 12, and 15 days after infection. Significantly more virus was detected in the brains of mice infected with IFN(−) virus as compared to mice infected with the IFN(+) virus. (C and D) Despite lower amounts of virus, mice infected with IFN(+) virus had a significantly greater percentage of activated (CD44hi CD62Llo) CD8+ T cells at 12 dpi. One representative mouse from each group is shown (C) and also the average of 5 mice per group for each time point (D). Horizontal lines indicate the average value and (*) indicate a p value of < 0.002.