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. 2009 Feb 27;4(2):e4631. doi: 10.1371/journal.pone.0004631

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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T9 cells in suspension were incubated with 1 mM phloretin, 0.01 mM pimaric acid, 1 mM H₂O₂ or in a hypotonic PBS (0.9× PBS) at 37°C. At timed intervals, the samples were analyzed on the flow cytometer. All data from the experimental groups are significantly different (P<0.05) from the untreated cells (black circles). Panel A shows the time kinetics; Panel B analyzes the data at 120 minutes and demonstrates the effect of 0.05 µM r-iberiotoxin in preventing cell swelling. Panel C shows that T9 cells swell in response to CO saturated media. All the experimental treatments in Panel A were significantly different (P<0.05) from the control cells. The asterisks in Panels B and C indicate a significant difference (P<0.05) between the treated cells and their respective controls.