Figure 7. BK channel activators cause a loss of intracellular ATP with the T9 cells.

T9 cells were cultured in either 1 mM phloretin, 0.01 mM pimaric acid, 0.05 µM r-iberiotoxin, 1 mM H₂O₂ or 0.1 mM DNP in 96 well plates. After 1, 16 or 24 hours the cells were lysed and the amount of ATP was measured using a luciferase/luciferin-based assay. The asterisks indicate a significant difference (P<0.05) of the experimental values from those results of either the untreated or iberiotoxin treated cells.