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HF diets increase protein tyrosine phosphatase 1 B (PTP1B) protein within the mediobasal hypothalamus. Mediobasal hypothalami were collected from rats described in Fig. 1, total protein was extracted, and levels of PTP1B protein were measured via Western Blot. Animals consuming a HF diet had significantly higher levels of PTP1B protein compared with rats consuming a LF diet (P = 0.003).
