Table 1.

Effect of added methionine and GAA on hepatocyte SAM and SAH levels and on the SAM-to-SAH ratio

	GAA	Control	Methionine
SAM	−	63.4±13.1	167.9±24.4*
SAH	−	10.2±2.4	38.5±3.7*
SAM-to-SAH	−	6.3±1.1†	4.4±0.8*
SAM	+	54.7±6.7	156.5±17.4*
SAH	+	12.6±2.7	42.6±5.4*
SAM-to-SAH	+	4.5±0.8	3.7±0.6

Data are expressed in nanomoles per gram wet mass of liver cells as means ± SD; n = 4 hepatocyte preparations. Hepatocytes were incubated for 60 min at 37°C and incubated 0.5 mM methionine and 125 μM guanidinoacetate acid (GAA). P < 0.05.

^*Significant difference from the control;

^†significant difference from corresponding incubations with GAA. SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine.