Fig. 1.

Hypoxia in adipose tissue of ob/ob mice. A: Po₂ in epididymal fat pads of lean and ob/ob mice was determined with an oxygen meter equipped with a fiber optic oxygen sensor. The assay was conducted in mice at 6, 9, and 12 wk in age. Fat composition and body weight of the mice were determined (n = 3). B: hypoxia response gene expression in epididymal fat of ob/ob mice was determined in quantitative RT-PCR. The assay was conducted in mice at 6 and 12 wk in age (n = 3). C: protein levels in epididymal fat of lean and ob/ob mice were examined in a Western blot (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001 vs. lean mice; #P < 0.05, ##P < 0.01 vs. 6-wk-old (6w) mice.