Fig. 6.
Hypoxia regulation of lipid metabolism. A: lipolysis was evaluated with glycerol release into culture medium of 3T3-L1 adipocytes. The assay was conducted after exposure to hypoxia or TNF-α (20 ng/ml) for 24 h (n = 4). Fresh 3T3-L1 adipocytes were cultured in conditioned medium (CM) made from supernatant of 3T3-L1 adipocytes treated by normoxia or hypoxia for 24 h. The net increase in glycerol level was obtained by comparing glycerol concentrations before and after 24-h culture in CM. B: [1-14C]palmitic acid uptake was performed in 3T3-L1 adipocytes after hypoxia treatment for 24 h (n = 3). C: FFA in femoral artery of lean rats. The femoral artery was clamped to block blood flow for 15 min to generate a hypoxia response in vivo. Blood samples were collected from the femoral vein before surgery and after clamp to determine the change in FFA (n = 11). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.