TABLE 1. Elongation efficiencies on the single-stranded RNA and hairpin RNA scaffold constructs at 3 μM and 300 nM scaffold-RNAP concentrations.

S.E. of all measurements were ±5%. The ATP concentration was 20 μM.

Scaffold	Percent (%) extended to position +3 with ATP		Percent (%) not extended in the presence of ATP
	3 μM	300 nM	3 μM	300 nM
ss-1	64	69	7	7
Hp-1	58	42	16	32
Hp-2	48	28	26	54
Hp-3	25	1.3	44	84