Abstract
A semisynthetic vaccine to Streptococcus pneumoniae type 3 has been developed. The hexasaccharide [leads to 3)GlcpA beta(1 leads to 4)Glcp beta(1 leads to]3 was isolated from a partial acid hydrolysate of the capsular polysaccharide S3. It was coupled to stearylamine by reductamination with NaCNBH3 and then incorporated into liposomes. These haptenated liposomes were tested for immunogenicity in mice. They induced protection to a lethal dose (25 50% lethal doses) of S. pneumoniae type 3 and gave rise to immunoglobulin M antibodies. No cross-protection was observed against S. pneumoniae type 11.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baker P. J., Stashak P. W., Prescott B. Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells. Appl Microbiol. 1969 Mar;17(3):422–426. doi: 10.1128/am.17.3.422-426.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campbell J. H., Pappenheimer A. M., Jr Quantitative studies of the specificity of anti-pneumococcal polysaccharide antibodies, types 3 and 8. I. Isolation of oligosaccharides from acid and from enzymatic hydrolysates of S3 and S8. Immunochemistry. 1966 May;3(3):195–212. doi: 10.1016/0019-2791(66)90184-4. [DOI] [PubMed] [Google Scholar]
- Kamerling J. P., Gerwig G. J., Vliegenthart J. F., Clamp J. R. Characterization by gas-liquid chromatography-mass spectrometry and proton-magnetic-resonance spectroscopy of pertrimethylsilyl methyl glycosides obtained in the methanolysis of glycoproteins and glycopeptides. Biochem J. 1975 Dec;151(3):491–495. doi: 10.1042/bj1510491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snippe H., van Houte A. J., van Dam J. E., De Reuver M. J., Jansze M., Willers J. M. Immunogenic properties in mice of hexasaccharide from the capsular polysaccharide of Streptococcus pneumoniae type 3. Infect Immun. 1983 Jun;40(3):856–861. doi: 10.1128/iai.40.3.856-861.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uemura K., Nicolotti R. A., Six H. R., Kinsky S. C. Antibody formation in response to liposomal model membranes sensitized with N-substituted phosphatidylethanolamine derivatives. Biochemistry. 1974 Apr 9;13(8):1572–1578. doi: 10.1021/bi00705a003. [DOI] [PubMed] [Google Scholar]
- Wood C., Kabat E. A. Immunochemical studies of conjugates of isomaltosyl oligosaccharides to lipid. I. Antigenicity of the glycolipids and the production of specific antibodies in rabbits. J Exp Med. 1981 Aug 1;154(2):432–449. doi: 10.1084/jem.154.2.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Houte A. J., Snippe H., Willers J. M. Characterization of immunogenic properties of haptenated liposomal model membranes in mice. I. Thymus independence of the antigen. Immunology. 1979 Jun;37(2):505–514. [PMC free article] [PubMed] [Google Scholar]