Figure 3. The effect of oxidative stress on epimastigotes previously incubated in PBS (PBS), L-proline 3 mM in PBS (PRO), and 3 mM Glucose in PBS (GLC), were treated or not with 0.5 mM T4C (PBS+T4C, PRO+T4C and GLC+T4C).

After washing, the parasites were incubated for 3 h in PBS and stressed with 160 µM H₂O₂. The cells viability was evaluated by MTT assay.