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Thin-layer chromatography of tyrosine-sulfate isolated from human trypsinogens after alkaline hydrolysis. The silica plate was developed using 80 % ethanol as solvent. The plate was then air-dried, sprayed with ninhydrin solution and heated with a hair-dryer. See text for experimental details. Tyrosine sulfate (Tyr-S) and (Tyr-P) standards were co-loaded with the trypsinogen-derived samples where indicated.
