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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: Biochemistry. 2009 Feb 24;48(7):1498–1507. doi: 10.1021/bi8020108

Figure 2.

Figure 2

7-deazaguanosine at the cleavage site reduces ribozyme activity. Plots of kobs with varying concentrations of MgCl2. (A) Comparison of wild type (WT) (■) and G1(7DG) substrates (●). (B) WT (■) and G2(7DG) (▼) substrates. (C) G1(7DG) (●) and G1G2(7DG) substrates (▼). The cleavage reactions were performed at 37 °C in 25 mM Tris buffer pH 7.0. Each point is the average of three independent experiments and the standard deviation is shown as error bars. Data were fit to equation 2 described in methods. The resulting kinetic parameters are provided in Table 1.