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. Author manuscript; available in PMC: 2010 Feb 24.
Published in final edited form as: Biochemistry. 2009 Feb 24;48(7):1498–1507. doi: 10.1021/bi8020108

Table 1.

Single turnover kinetics parametersa

kmax (min−1) Kd,Mg2+ (mM) αMg
Substrates pH 5.0 pH 7.0 pH 5.0 pH 7.0b pH 5.0 pH 7.0
WT 2.0 ± 0.2 7.0 ± 0.2 30 ± 8 2.1 ± 0.1 0.78 ± 0.08 1.6 ± 0.1
G1(7DG) 1.2 ± 0.1 1.1 ± 0.1 60 ± 22 1.9 ± 0.3 0.78 ± 0.09 1.7 ± 0.4
G2(7DG) NDc 6.2 ± 0.4 ND 3.0 ± 0.6 ND 1.3 ± 0.3
G1G2(7DG) ND 0.62 ± 0.01 ND 1.7± 0.1 ND 2.3 ± 0.2
a

The values and standard errors were obtained by non-linear least-squares fitting in KaleidaGraph (Synergy software).

b

Kd,Mg2+ should be treated as apparent Kd,Mg2+ when the Hill coefficients are greater than 1.

c

Not determined.