TABLE 2.

GENE EXPRESSION STUDIES USING CULTURED AIRWAY SMOOTH MUSCLE CELLS

Author (Reference No.)	Source of Cells	Exposures	Method of Gene Expression Profiling	Findings
Lee and coworkers (28)	Commercially available human airway smooth muscle cells	IL-13 (100 ng/ml)	Affymetrix Genechip HuGeneFL arrays coding for ∼6,500 human genes	204 Genes induced two-fold, including smooth muscle MHC, phospholipase A2, diacyglycerol kinase δ, and IL-13Rα1
Syed and coworkers (30)	3 Lung transplant donors	IL-13 (10–100 ng/ml)	Research Genetics and Incyte Genomics cDNA microarrays coding for 8,159 genes	A large number of genes induced 1.5-fold or by FDR analyses (total no. not given), including VCAM1, IL-13Rα2, Tenascin C, and histamine receptor H1
		IL-13R130Q*
Jarai and coworkers (27)	2 Patients undergoing lung resection	IL-13†	Affymetrix HG-U95Av2 microarrays coding for ∼12,500 genes	H1 receptor and tenascin were highly induced by IL-13
		TGF-β†
		IL-1β†
McGraw and coworkers (29)	Wild-type and transgenic mice overexpressing β2-adrenergic receptor	None	University of Cincinnati Genomic and Microarray Laboratory array coding for 8,734 cDNAs	319 Genes induced
				164 Repressed (including phospholamban)

Definition of abbreviations: FDR = false discovery rate; IL-13Rα1 = IL-13 receptor α1; MHC = myosin heavy chain; RT-PCR = reverse transcription–polymerase chain reaction; TGF = transforming growth factor; VCAM = vascular cell adhesion molecule.

^*A naturally occurring isoform of IL-13.

^†All at a dose of 10 ng/ml.