Figure 3.

Treatment of cells with 8Br-cADPR results in attenuated [Ca²⁺]_i responses to agonists in control and cytokine-treated human airway smooth muscle (HASM) cells. Fura-2/AM-loaded control and cytokine-treated HASM cells were incubated with 8Br-cADPR and [Ca²⁺]_i responses to agonists were measured. The net [Ca²⁺]_i values in the presence of 8Br-cADPR in control and cytokine-treated cells were compared with those of the corresponding controls (in the absence of 8Br-cADPR). Net [Ca²⁺]_i responses to acetylcholine, bradykinin, and thrombin in control and cytokine-treated HASM cells, in the presence or absence of 8Br-cADPR, are shown. Panels on the right represent % inhibition of the [Ca²⁺]_i responses by 8Br-cADPR. Note significant (P ≤ 0.05) attenuation of [Ca²⁺]_i responses in the presence of 8Br-cADPR in control and cytokine-treated HASM cells. Data are from three different cell preparations. IL-1β = interleukin-1β; TNF-α = tumour necrosis factor-α; IFN-γ = interferon-γ. Reprinted by permission from Reference 48.