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. Author manuscript; available in PMC: 2009 Dec 26.
Published in final edited form as: J Mol Biol. 2008 Oct 9;384(4):929–940. doi: 10.1016/j.jmb.2008.09.077

Figure 7. Effect of IF3mt derivatives on the binding of [35S]fMet-tRNA to mitochondrial 28S subunits in the absence of mRNA.

Figure 7

(A) Reaction mixtures contained 160 nM IF2mt and increasing levels of IF3mt or its C-domain derivatives at a constant level of 28S subunits (50 nM). In the absence of IF3mt 0.1pmol of fMet-tRNA were bound to the 28S subunits by IF2mt in the reaction mixture. The estimated error of this experiment is ± 10%.