Table 2.
Validation sample set | Sample | Control | P | FP | FDR |
I | SP/RP (UL) | SP/RP (IS) | 22 | 1 | 0.045 |
II | SA/SB (UL) | SA/SB (IS) | 8 | 2 | - |
SB/SC (UL) | SB/SC (IS) | 0 | 1 | - | |
SC/SA (UL) | SC/SA (IS) | 1 | 0 | - | |
Average | 3.0 | 1.0 | 0.33 | ||
III | SP/RP (UL) | SA/SB (UL) | 22 | 8 | - |
SB/SC (UL) | 22 | 0 | - | ||
SC/SA (UL) | 22 | 1 | - | ||
Average | 22 | 3.0 | 0.14 | ||
IV | SP/RP (IS) | SA/SB (IS) | 1 | 2 | - |
SB/SC (IS) | 1 | 1 | - | ||
SC/SA (IS) | 1 | 0 | - | ||
Average | 1.0 | 1.0 | 1.0 | ||
V | SP/RP (UL) | Inj.1 vs Inj.2 (UL) of SP, SA, SB, and SC | 22 | 0 | 0.00 |
Sample set I represents the target of validation. The parameters used for FDR calculation were: 2-fold change, 4 MPSP, and t-test (p < 0.05). P, positives. FP, false positives. '-', not calculated. See text for more details.