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Kit messenger is unchanged after TGF-β treatment. A, RT-PCR. Skin-derived mast cells were treated with TGF-β for up to 6 h. Kit mRNA was assessed by RT-PCR. Representative data from one of three experiments are shown. B, Real-time RT-PCR. RNA from skin mast cells treated with TGF-β for 24 h was isolated and subjected to real-time RT-PCR using the SYBR Green method. Data are expressed as mean ± SE of three independent experiments with p = 0.43.
