Table 2.
Direct comparison between the two mutants (C99I45F versus C99V50F) showed CRABP1 as up-regulated in C99I45F (Aβ42/Aβ40↑) whereas neurofilaments were not differentially expressed
| Name | Fold change C99I45F/C99V50F | P-value | ||
| Transcriptomics | Proteomics | Transcriptomics | Proteomics | |
| CRABP1 | 2.3 | 2.8 | 0.188 | 0.059 |
| NEF3 | 1.1 | -1.1 | 0.790 | 0.56 |
| NEFL | 1.1 | -1.1 | 0.640 | 0.51 |
| INA | 1.1 | -1.1 | 0.679 | 0.24 |
Direct comparison of C99I45F and C99V50F (baseline experiment, C99V50F) revealed effects mediated by an altered Aβ42/Aβ40 ratio for CRABP1 by a fold change distinctly deviating from 1.0, and an effect mediated by C99 for neurofilament 3 (NEF3), neurofilament light polypeptide 68 kDa (NEFL) and internexin neuronal intermediate filament protein alpha (INA) by a fold change close to 1.0, because C99 was approximately equally expressed in both mutants. Significance was determined performing an unpaired t-test for the direct comparison of both mutants. As to be expected, p-values were high for not differentially expressed genes [62,63].