Figure 4. Conserved regions B and C encode transcriptionally active sequences.

Luciferase reporter gene (pGL3) constructs containing conserved region B, B and C, or vector alone were transiently cotransfected into EL4 or A20 cells with a constitutive renilla luciferase expression vector. Following 24 hours, cells were lysed and assayed for luciferase activity as described in Materials and Methods. Samples were normalized to the renilla luciferase activity and the average values from three independent experiments were plotted as fold over vector alone.