Table 1.
List of oligonucleotides primers used for qRT-PCR and to generate promoter 1 and 2 deletion constructs.
| Primers for qRT-PCR (5’ to 3’) | ||
|---|---|---|
| 18S | F | ATGCTCTTAGCTGAGTGTCCCG |
| R | ATTCCTAGCTGCGGTATCCAGG | |
| All msrAs | F | GCTTCGGCCCCATCACTAC |
| R | GCCATTGGGGTTCTTGCTCA | |
| msrA2-3 | F | GAGAGGCAGAGGGAGAGC |
| R | TCAAGCCGAGAAAGCAGA | |
| Primers to generate promoter constructs | ||
| Promoter 1 | ||
| −1919 | F | AGTTTCACCCCGAAACCATCC |
| −1232 | F | CATGCCCAACTACCTTAAATACCTT |
| −408 | F | ACTGCCTTAATGGTGCTGTTGGTG |
| −24 to−1 | R | AACTTCGTCCCGAGAGAACCTCAG |
| Promoter 2 | ||
| −1979 | F | AAGAGTACATTTATCCCATTTT |
| −1544 | F | GCTTCTCCAATGCTGCTTTCA |
| −971 | F | AGAACGTGGCAGAATCAAGGACTA |
| −499 | F | GACATTTCTGGGCATACGGTTTTA |
| −311 | F | CATATGGACATTTCTAGGGAACAGA |
| −208 | F | AGGATACCATGAAATCTGTAAGGTGA |
| −151 | F | GATTTGGGTACCGTACCACGGTT |
| −112 | F | TAATTGATCCGCAAAGGACATCT |
| −23 to−1 | R | TTGCCATACTGGGAGCAAAAGAGT |